By Joel Rothman, Andrew Singson
The moment a part of an up-to-date variation of the vintage Methods in mobile Biology, Volume forty eight, this publication emphasizes assorted tools and applied sciences had to examine C. elegans, either as an built-in organism and as a version method for examine inquiries in cellphone, developmental, and molecular biology, in addition to in genetics and pharmacology. by way of directing its viewers to tried-and-true and state of the art recipes for study, this complete assortment is meant to lead investigators of C. elegans for years to come.
- Diverse, up to date suggestions coated can be priceless to the broadening neighborhood of C. elegans researchers for years to come
- Chapters written by means of leaders within the field
- Tried and precise tools bring busy researchers a one-stop compendium of crucial protocols
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Additional resources for Caenorhabditis elegans : cell biology and physiology
Development. 134, 2227–2236. Zucker, R. , and Price, O. T. (1999). Practical confocal microscopy and the evaluation of system performance. Methods. 18, 447–458. CHAPTER 2 Immunofluorescence Microscopy Diane C. Shakes*, David M. Miller IIIy and Michael L. Nonetz * Department of Biology, College of William and Mary, Williamsburg, Virginia, USA y Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee, USA z Department of Anatomy and Neurobiology, Washington University Medical School, St.
Immunological reagents are also uniquely suited for distinguishing specific posttranslational modified forms of a protein. On the other hand, desired antigens are not always immunogenic, or, in cases in which a strong immune response is induced, the resultant antibodies may lack specificity and label inappropriate targets. In these cases, FP transgenes provide an 42 Diane C. Shakes et al. alternative approach. In addition, FP transgenes can be specifically engineered to be expressed and thus analyzed within a subset of their normal expression domain.
Worms are dissected in the drop (not shown). (B) The Vaseline ring slide is inverted and pressed onto the coverslip so that the Vaseline makes contact all around the circle and the liquid droplet contacts the slide. The assembly should remain coverslip side-down during preparation and mounting on an inverted compound microscope. direction of the embryos. Alternately, simply draw a ring around the embryos with a marker (on the slide, not coverslip) to aid you in centering the sample on the compound microscope.