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Basic and Clinical Science Course, 2008-2009, Section 4: by Debra J. Shetlar, Patricia Chevez-Barrios, Sander Dubovy,

By Debra J. Shetlar, Patricia Chevez-Barrios, Sander Dubovy, Robert H. Rosa, Jr, Nasreen Syed, Matthew W. Wilson, Ron W. Pelton, Jacob Pe'er

Discusses advances within the analysis and category of tumors because it courses the reader via a logical, tissue-specific series that levels from topography via sickness procedure to normal and differential prognosis. Many new colour pathologic and medical pictures and diagrams spotlight this most recent revision. Covers wound fix; specimen dealing with, together with processing and marking; and diagnostic innovations. additionally encompasses a record for soliciting for ophthalmic pathologic session.

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Extra info for Basic and Clinical Science Course, 2008-2009, Section 4: Ophthalmic Pathology and Intraocular Tumors

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Fluorochrome-labeled specific antibodies bind to the surface of lymphoid cells, and a suspension of labeled CHAPTER 4: Special ................ : Procedures . 45 . 100 w~~ : W lasers I I ~r'po.. ~ D E T E C T o R S Figure 4-4 Flow cytometry analyzes particles or cells moving in single file in a fluid stream (a). Fluorochrome-labeled specific antibodies bind to the surface of cells, and a suspension of labeled cells is sequentially illuminated by a laser (b). As the excited fluorochrome returns to its resting energy level, a specific wavelength of light is emitted (c) that is sorted by wavelength (d) and received by a photodetector (e).

Histiocytes have eccentric nuclei and abundant eosinophilic cytoplasm. In some instances, histiocytes may take on the appearance of epithelial cells, with abundant eosinophilic cytoplasm and sharp cell borders, becoming known in the process as epithelioid histiocytes. Epithelioid histiocytes may form a ball-like aggregate known as a granuloma, the sine qua non for granulomatous inflammation. These granulomas may contain only histologically intact cells ("hard" tubercles, Fig 1-7), or they may exhibit necrotic centers ("caseating" granulomas, Fig 1-8).

Resolution limitations; cannot detect small aberrations 1. Need for dividing cells 2. Resolution limitations; cannot detect small aberrations 1. Localization in the lesion of positive cells 2. Fluorescent signal easily detected in fresh or fixed tissue 1. Known type and location of expected aberrations 2. Limited number of chromosomal loci at one time Quality snapfrozen tissue (optimal) and archival paraffinembedded tissue 1. Variable success rate of DNA extraction 2. Contamination with other nucleic acid material 1.

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