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Analytical Chemistry of Bacillus thuringiensis by Leslie A. Hickle, William L. Fitch

By Leslie A. Hickle, William L. Fitch

content material: Analytical chemistry of Bacillus thuringiensis : an outline / Leslie A. Hickle and William L. Fitch --
historic features of the quantification of the lively component percent for Bacillus thuringiensis items / George Tompkins, Reto Engler, Michael Mendelsohn, and Phillip Hutton --
Bioassay tools for quantification of Bacillus thuringiensis [delta]-endotoxin / Clayton C. Beegle --
Specificity of insecticidal crystal proteins : implications for commercial standardization / R. Milne, A.Z. Ge, D. Rivers, and D.H. Dean --
In vitro analyses of Bacillus thuringiensis [delta]-endotoxin motion / George E. Schwab and Paul Culver --
identity of entomocidal pollutants of Bacillus thuringiensis via high-performance liquid chromatography / Takashi Yamamoto --
Characterization of parasporal crystal pollutants of Bacillus thuringiensis Subspecies kurstaki lines HD-1 and NRD-12 : use of oligonucleotide probes and cyanogen bromide mapping / L. Masson, M. Bossé, G. Préfontaine, L. Péloquin, P.C.K. Lau, and R. Brousseau --
improvement of a high-performance liquid chromatography assay for Bacillus thuringiensis var. san diego [delta]-endotoxin / Leonard Wittwer, Denise Colburn, Leslie A. Hickle, and T.G. Sambandan --
Use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to quantify Bacillus thuringiensis [delta]-endotoxins / Susan M. Brussock and Thomas C. Currier --
Quantitative immunoassay of insecticidal proteins in Bacillus thuringiensis items / R. Gene Groat, James W. Mattison, and Eric J. French --
The light-scattering characterization of [delta]-endotoxin creation in inclusion our bodies / Fritz S. Allen, Betty J.M. Hannoun, Tammy B. Hebner, and Kathryn Nette --
Quantification of Bacillus thuringiensis insect keep an eye on protein as expressed in transgenic crops / Roy L. Fuchs, Susan C. MacIntosh, Duff A. Dean, John T. Greenplate, Frederick J. Perlak, Jay C. Pershing, Pamela G. Marrone, and David A. Fischhoff --
High-performance liquid chromatography research of 2 [beta]-exotoxins produced by means of a few Bacillus thuringiensis traces / Barry L. Levinson.

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Indiana Acad. Sci. 1988 (in press). ; Martouret, D. J. Invert. Pathol. 1965, 7, 105-08. M. Arch. Microbiol. 1982, 132, 159-62. Luthy, P. FEMS Microbiol. Lett. 1980, 8, 1-7. S. J. Invert. Pathol. 1974, 24, 365-73. , Jr. Appl. Environ. Microbiol. 1985,50,737-42. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1990. 4. MILNE ET AL. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. I. J. Bacteriol. 1977, 130, 375-83. M. Biochem. J. 1980, 187, 457-65. , Jr. Can.

Haider and Ellar (50) showed that for the strain aizawai ICI, the toxin generated by lepidopteran enzymes retains lepidopteran activity. This same toxin can be further processed by dipteran enzymes to produce a dipteran specific toxin. In this case, the removal of ~ 15 amino acids from the C-termmal end of the toxin fragment resulted in a shift in the host specificity. The importance of a characterized N-terminal site has been shown by Hofte et al. (51) using gene deletion and expression. They determined that toxicity was lost in a deletion between amino acid positions 29 and 37.

54. I. J. Bacteriol. 1977, 130, 375-83. M. Biochem. J. 1980, 187, 457-65. , Jr. Can. J. Microbiol. 1988,34,740-47. W. ; Permagon: Toronto, 1985; Vol. 4. p. 279. ; Kuriyama, K. Comp. Biochem. Physiol. 1983, 76B, 29-34. R. Can. J. Zool. 1989, 67, 864-68. R. Pharmac. Ther. 1981, 13, 257-83. ; Aizawa, K. Agric. Biol. Chem. 1986, 50, 575-80. Murphy, D. W. D. Thesis, University of California, 1973. ; Itai Biol. Chem. 1984, 48 Aronson, A. ; Dunn, P. Microbiol. Rev. 1986, 50, 1-24. ; Carey, P. ; Pozsgay, M.

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