Affinity chromatography by Sameh Magdeldin

By Sameh Magdeldin

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Recombinant protein G (cell surface protein) is cloned in Streptococcus while recombinant protein L is cloned from Peptostreptococcus magnus. Both protein A and G specifically bind the Fc region of IgG while protein L binds to the kappa light chains of IgG. g. Sepharose CL-4B; agarose crosslinked with 2,3dibromopropanol and desulphated by alkaline hydrolysis under reductive conditions), polyacrylamide, and magnetic beads (Grodzki & Berenstein, 2010; Hober et al, 2007; Katoh et al, 2007; Tyutyulkova & Paul, 1995).

G. from low stability, sensitivity to process conditions or from tendency to be inhibited by high concentrations of reaction components of some of these biocatalysts. , 2005; Guisan, 2006; Hernandez & Fernandez-Lafuente, 2011; Krajewska, 2004). Enzyme immobilization enables primarily the re-use or continuous use of the biocatalysts and it also substantially simplifies the manipulation with the biocatalyst and the control of the reaction process. Also the separation of the enzyme from the reaction mixture is Affinity Interactions as a Tool for Protein Immobilization 31 significantly easier and protein contamination of final product is minimized.

Hage DS, Anguizola J, Barnaby O, Jackson A, Yoo MJ, Papastavros E, Pfaunmiller E, Sobansky M, Tong Z (2011) Characterization of drug interactions with serum proteins by using high-performance affinity chromatography. Curr Drug Metab 12: 313-328 Hage DS, Austin J (2000) High-performance affinity chromatography and immobilized serum albumin as probes for drug- and hormone-protein binding. J Chromatogr B Biomed Sci Appl 739: 39-54 Hermanson GT, Mallia AK, Smith PK (1992) Immobilized Affinity Ligand Techniques, New York: Academic Press.

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